Eurofins Biomnis code

GIST

Synonyms

• solid tumor
• companion test
• NGS
• oncology
• targeted therapy

Clinic significance

Testing for KIT, PDGFRA and BRAF mutations thus has diagnostic and/or prognostic and/or theranostic value.

Prenalytics

• Tumoral block included in paraffin or slides (6 slides 5µm) or DNA extract
• Ambient temperature

Further information

• Attach the anatomopathological report
• Use the specific request form B9-INTGB: Oncology-Solid tumors
• The use of the S25UK transport bag is Mandatory.

Specific equipment available

S25 : Envelope Genetics and Molecular Oncology

Documents to download

• Specific form (B9-INTGB)
• Management of solid tumours in Biopathology (DS5-INTGB)
• Digestive system Panel form (DS84-INTGB)

Methodology

Targeted sequencing of cancer genes (NGS) : -Library : AmpliSeq Library PLUS for Illumina Custom Panel V1 - Amplicon-based targeted library preparation method -NGS Platform : MiSeq Illumina - Flowcell : microV2 (2x150pb) - Paired-end reads -Analysis software : Miseq control Software version 2.6.2.1 /SeqOne Platform v1.2 - CE Pipeline version : SomaVar v1.4 CE -Limit of detection : 5% -Minimum depth accepted : 500x -Human genome version : Hg19

Turnaround time

10 days (Results may require an extended turnaround time, one week, depending on the confirmation tests required by Sanger sequencing)