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Herpes virus type 8 (HHV8) - direct diagnosis - PCR

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Eurofins Biomnis code

HV8BM

Synonyms

  • HHV-8 - PCR
  • KSHV - PCR
  • HHV-8 - molecular diagnosis

Clinic significance

HHV8 is a virus belonging to the Herpesviridae family. Its distribution follows a North-South gradient. Transmission is salivary in high-prevalence countries, with primary infection occurring in childhood. In low-prevalence countries, primary infection occurs in adulthood through sexual transmission. Primary infection is asymptomatic and usually remains unrecognised. The pathogenesis of HHV8 is based on its oncogenic nature. It is responsible for three tumour diseases: Kaposi's disease, Castelman's disease and primary serous lymphoma. These diseases most often occur in the context of immunodepression. Diagnosis is based on the macroscopic appearance of the lesions, testing for the HHV8 genome in blood, biopsies or biological fluids of interest, and histological analysis of the lesions. Testing for the HHV8 genome in blood can be negative in Kaposi's disease.

Prenalytics

  • 1 mL
  • Biopsy (of a tumor, skin or organ lesion), puncture fluid (peritoneal, pericardial, pleural fluid, bone marrow (EDTA)
  • Refrigerated

Further information

  • The use of the S14UK transport bag is Mandatory.
  • Samples (other than swabs) must be sent as they are, in sterile bottles, without transport medium.
  • Swab samples require the use of a transport medium (viral) supplied in kit K1.

Specific equipment available

K1: Virus screening kit by culture or PCR

Documents to download


Methodology

Real-time PCR

Turnaround time

6 days


Pathologist(s) in charge
Biomnis Lyon
Specialty
Infectious
Contact(s)
Dr Anaïs SOARES
Dr Marie HERVO
Dr Thomas BLANCHOT
Phone(s)
+334 72 80 73 99
+334 72 80 73 99
+334 72 80 73 99