Eurofins Biomnis code

MOHC1

Synonyms

• Conventional acquired cytogenetics
• Acquired karyotypes

Clinic significance

Indications:
- Myeloid neoplasia:
Myeloproliferative neoplasia (MPN): CML, CNL, PV, MFP, TE
Mastocytosis
Myeloid/lymphoid neoplasia with eosinophilia
Myelodysplastic/myeloproliferative neoplasia: CML, CMML, JMML
Myelodysplastic syndromes
- Mature lymphoid neoplasia:
Mature B-cell neoplasia: CLL, B-cell prolymphocytic leukemia, MZL, Hairy cell leukemia, Waldenström's macroglobulinemia, lymphoplasmacytic lymphoma, follicular lymphoma, GCPL, Burkitt's, high-grade B-cell lymphoma
Monoclonal gammopathy of undetermined significance (MGUS), multiple myeloma
Mature T- and NK-cell neoplasia: prolymphocytic T-cell leukemia, LGL, hepatosplenic T-cell lymphoma, Sezary syndrome/mycosis fungoides, angioimmunoblastic T-cell lymphoma, anaplastic large cell lymphoma
- Acute leukemias:
Acute myeloid leukemias and associated neoplasia: AML with recurrent genetic abnormalities, AML with abnormalities related to myelodysplasias, treatment-related myeloid neoplasias, AML NOS
Acute leukemias of ambiguous lineage
B-precursor lymphoblastic lymphoma/leukemia
T-precursor lymphoblastic leukemia/lymphoma
Chimerism

Prenalytics

• 5 mL Heparinised whole blood, 2 mL Bone marrow, lymph nodes
• Ambient temperature

Further information

• Attach:
  - the clinical and therapeutic data
  - the results of the FBC - Platelets
  - the bone marrow report
  - the immunophenotyping report
• Bone marrow: 1 heparinised syringe or medullary puncture transferred into a lithium heparinate tube ,Lymph node: sterile bottle
• Cytogenetic study in the context of Myeloma: must be performed on bone marrow (not on peripheral blood). Exception: Plasma cell leukaemia: peripheral blood or bone marrow.

Specific equipment available

S9L: Envelopes yellow for karyotypes LYON

Documents to download

• Specific request form B8-INTGB
• DS2-INTGB - Pathological diagnosis and follow up of patients with Chronic Myeloid Neoplasms
• DS3 -INTGB - Acute leukemia
• DS62- Mature lymphoid noeplasm

Methodology

Conventional cytogenetics: Culture + chromosome analysis

Turnaround time

Time according to indication: - AL: 1 week - CMN and MDS: 2 weeks - LPD and myeloma: 3 weeks