Eurofins Biomnis code

PCRUN

Synonyms

• DNA bacterien
• ARN ribosomal 16S
• panbacterien
• 16S rRNA
• universal PCR
• NGS
• Next Generation Sequencing

Clinic significance

16S PCR is a molecular biology technique that can be used to detect and identify any bacterial genome in a biological sample. 16S PCR is ""universal"" because it targets a genomic sequence common to all bacteria: a gene coding for 16S ribosomal RNA.
The technique consists of 16S PCR detection, enabling amplification of this gene, and sequencing of the PCR product using NGS (Next Generation Sequencing) technology.
16S PCR can be performed directly on clinical samples from sites that are normally sterile (puncture fluids, heart valves, organ biopsies, etc.) or on isolated bacterial strains.
This method is particularly suitable for identifying slow-growing bacteria and bacteria that are difficult or impossible to culture. It is also extremely useful for biological samples taken from patients previously treated with antibiotics.
Universal 16S PCR is a molecular biology tool that can be useful in diagnosing a variety of infections (endocarditis with negative blood cultures, osteoarticular infections, etc.) when the cytobacteriological examination is negative and there is a strong clinical suspicion of infection. Because of the sensitivity of universal PCR techniques, a negative 16S PCR result cannot formally rule out infection.
Unlike pathogen-specific or ""targeted"" PCRs, 16S PCR is ""pan-bacterial"": it can detect any bacteria present in a sample. However, the sensitivity of a specific PCR is greater than that of a universal PCR.
Where there is a strong clinical presumption of infection by a bacterial species or genus, targeted research using a specific PCR should therefore be the first choice.
NGS technology makes it possible to identify several bacteria present in a biological sample at the same time.
Universal 16S PCR makes it possible to determine the bacterial origin of complex clinical pictures and to prescribe antibiotic therapy adapted to the identified germ. It is an essential technique in the diagnostic and therapeutic strategy for many infections.
The prescription of this test must be accompanied by the patient's clinical and therapeutic context.

Prenalytics

• Puncture fluids (joint fluid, ascites fluid, pericardial fluid, pleural fluid), osteoarticular samples, heart valve, deep eye samples (aqueous humor, vitreous humor), biopsy, CSF, bacterial strain
• Refrigerated

Further information

• The use of the S14UK transport bag is Mandatory.

Documents to download

• Specific request form (B18-INTGB)

Methodology

NGS sequencing

Turnaround time

8 days