NGS "LUNG1" panel: AKT1, ALK, BRAF, DDR2, EGFR, HER2, FGFR1, FGFR2, FGFR3, KIT, KRAS, MAP2K1, MET, NRAS, PDGFRA, PIK3CA, PTEN, STK11, TP53
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POUM2
Synonyms
- companion test
- targeted therapy
- oncology
- NGS
- solid tumor
Clinic significance
The NGS "LUNG2" panel approach expands the range of tested mutations in the genes
implicated in resistance mechanisms for targeted therapies, emerging markers for targeted therapies or immunotherapy response, or potential targets for clinical research. This approach should be complemented by targeted FISH tests.
Prenalytics
- Tumoral block included in paraffin or slides (6 slides 5µm) or DNA extract
- Ambient temperature
Further information
- Attach the anatomopathological report
- Use the specific request form B9-INTGB: Oncology-Solid tumors
- The use of the S25UK transport bag is Mandatory.
Specific equipment available
S25 : Envelope Genetics and Molecular Oncology
Documents to download
Methodology
Targeted sequencing of cancer genes (NGS) : -Library : AmpliSeq Library PLUS for Illumina Custom Panel V1 - Amplicon-based targeted library preparation method -NGS Platform : MiSeq Illumina - Flowcell : microV2 (2x150pb) - Paired-end reads -Analysis software : Miseq control Software version 2.6.2.1 /SeqOne Platform v1.2 - CE Pipeline version : SomaVar v1.4 CE -Limit of detection : 5% -Minimum depth accepted : 500x -Human genome version : Hg19
Turnaround time
10 days (Results may require an extended turnaround time, one week, depending on the confirmation tests required by Sanger sequencing)