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Glucagon test

List of dynamic tests
Purpose

To measure residual insulin secretion after stimulation (notably in a subject with nascent diabetes).

Subject preparation

Fasting overnight and at rest.

Product

Glucagon Novo®.

Dose

1 mg by slow IV administration.

Protocol
  • Blood sample (1 dry tube and 1 fluoride tube) for each time intervals: –15 min (T-15) and 0 (T0).
  • Slow IV injection of the correct dose of glucagon.
  • Blood samples (1 dry tube and 1 fluoride tube for each time interval) after:
    • Short protocol: 10 min (T+10)
      or
    • Full protocol: 3 (T+3), 6 (T+6) and 15 (T+15) min:
Samples

After centrifugation, draw off plasma and serum. Freeze serum and store plasma at +4°C.

Assay
  • Peptide C.
  • Insulin.

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