HomeServicesDynamic TestsSynacthen® test (rapid or immediate)

Synacthen® test (rapid or immediate)

List of dynamic tests

To investigate adrenocortical function after stimulation with an ACTH analogue.


Tetracosactide (Synacthen®, 0.25 mg / 1 ml vial).

  • Adults: 0.25 mg (1 vial).
  • Children: 0.25 mg/m2 body surface area (do not exceed 0.25 mg).
  • Fasting for 12 hours and at rest for 30 min.
  • Sample (dry or EDTA tube) at time interval 0 (8 a.m.) (T0).
  • IM injection of the correct dose of Synacthen®.
  • Samples (dry or EDTA tubes) according to the doctor’s instructions or T+30 and T+60 min after the Synacthen® injection.

After centrifugation of the samples, store the serum or plasma at +4°C.


Cortisol and/or: 17-hydroxyprogesterone, 21-deoxycortisol, 11-deoxycortisol, 17-hydroxypregnenolone, dehydroepiandrosterone, androstenedione, 11-deoxycorticosterone, aldosterone.

  • Allergy.

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