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HomeServicesDynamic TestsUrea breath test (Helicobacter pylori)

Urea breath test (Helicobacter pylori)

List of dynamic tests
Purpose

In-vivo diagnosis of Helicobacter pylori, notably the monitoring of eradication therapy

Product

Helikit® (13C urea: 75 mg ; citric acid : 1.4 g), Mayoly-Spindler Laboratories
(Other Kit: Helicobacter test Infai® ( 13C urea: 75 mg powder for oral solution), Infai laboratories)

Precautions
  • No antacids or gastrointestinal protectants in the last 24 hours.
  • Discontinue all antibiotics at least four weeks before testing.
  • Discontinue proton-pump inhibitors at least 2 weeks before testing.
Protocol
  • Subject having fasted for at least 6 hours and at rest; no eating, drinking or smoking during the challenge.
  • Clearly mark four sample tubes (2 tubes T0 and 2 tubes T30) (only use 10 ml Exetenair® tubes).
  • Heli-Kit
    • Dissolve the contents of the kit’s citric acid sachet in 200 ml water.
    • Give the subject 100 ml of this solution to drink and keep the rest.
  • Helicobacter test®
    • Give to the subject 200ml of orange juice (100% pure juice)
    • Dissolve the contents of the kit’s citric acid sachet in 200 ml water.
    • Give the subject 100 ml of this solution to drink and keep the rest.
    • Open the two T0 tubes by unscrewing the cap.
    • Get the patient to blow gently into one of the T0 tubes for at least 15 seconds, through the first straw. Continue blowing until condensation appears on the walls of the tube. Remove the straw while still blowing and quickly close the tube.
    • Repeat with the second T0 tube.
  • Heli-Kit
    • Dissolve the contents of the kit’s urea sachet in the rest of the citric acid solution (100 ml).
  • Helicobacter test®
    • Dissolve the contents of the kit’s urea sachet in 30ml of water
    • Get the subject to swallow the urea solution and start the timer.
    • Wait for 30 minutes.
    • Open the two T30 tubes by unscrewing the cap.
    • Get the patient to blow gently into one of the T30 tubes for at least 15 seconds, through the second straw. Continue blowing until condensation appears on the walls of the tube.
    • Remove the straw while still blowing and quickly close the tube.
    • Repeat with the second T30 tube.
    • Re-seale tubes quickly and securely.
    • Insert the tubes in a sealed red bag (ambient temperature), fill in the order form and include it.
Results

Presence / absence

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