Eurofins Biomnis code

BRAF

Specialty

Genetics

Clinical significance

The BRAF NGS panel involves mutational analysis of the BRAF gene. This mutation is reported in more than 95% of cases of Hairy Cell Leukaemia and represents a very useful diagnostic criterion in association with a targeted immunophenotypic analysis.

Preanalytics

• 5 ml EDTA whole blood or 2 ml EDTA bone marrow or DNA extracted : (200ng of DNA minimum)
•   Ambient temperature

• A tube specifically for this analysis : No

Further information

Samples to be collected from Monday to Friday
Sample must be refrigerated if transport is > 48 hours
Use the specific request form B8-INTGB: Malignant blood disorders
Attach:
- the results of the FBC- platelets
- the bone marrow report
- the immunophenotyping report
- Results of lymph node histology (if performed)

Specific equipment available

• S9L: Envelopes yellow for karyotypes LYON

Documents to download

• Specific request form B8-INTGB
• Panel Form(DS120-INTGB)

Methodology

Method: Targeted sequencing of genes (NGS). - Library: Library Preparation Enzymatic Fragmentation Kit 2.0 Twist - NGS Platform: NovaSeq Illumina (2x150pb) - paired-end sequencing - Analysis software: Demultiplexage BCLconvert V3.10.5 / VarSome Pipeline version 11.8 (CE-IVD)

Turnaround time

10 days