Chikungunya - direct diagnosis - PCR

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Eurofins Biomnis code

CHKBM

Specialty

Infectious


Clinical significance

The Chikungunya virus (from the Swahili word for ""bent walking"", because of the severe joint pain it causes) is an alphavirus of the Togaviridae family transmitted by the bite of mosquitoes of the Aedes genus. It circulates mainly in the intertropical zone (Africa, Asia and the Pacific) and in certain regions of Europe. The acute stage of the infection is characterized by a sudden onset of high fever, usually of short duration. This is typically accompanied by severe joint pain in the extremities of the limbs (wrists, ankles, phalanges) and, occasionally, a macular exanthema. The spontaneous course of the disease is usually favourable. Chronic forms exist, characterised by persistent, recurrent and sometimes disabling arthralgia. RT-PCR is indicated up to 7 days after the onset of clinical signs. Serological diagnosis can be carried out as early as 5 days after the onset of clinical signs, to detect seroconversion or IgM antibodies at the end of the first week of infection. Chikungunya is a reportable disease.

Preanalytics
  • A tube specifically for this analysis : No
Further information

Enclose the specific clinical information form (R28-INTGB : Dengue-Chikungunya-West Nile-Zika)
Sample to be collected up to 7 days after the clinical symptoms onset
The use of the S14UK transport bag is Mandatory.
.

Specific equipment available
  • S14: Special mycobacteria transport bags are to be used

Methodology

Real-time PCR

Turnaround time

3 days


Testing Laboratory

Biomnis Lyon