Eurofins Biomnis code

CMVBM

Synonyms

• CMV
• Cytomégalovirus
• Cytomegalovirus - CMV - detection PCR
• HHV-5
• Human Herpes Virus-5

Specialty

Infectious

Clinical significance

Cytomegalovirus (CMV) belongs to the Herpesviridae family. The virus is transmitted via saliva, urine, genital secretions, blood, transplanted organs, breast milk or through the placenta. Viral excretion is less intense during reactivations. In immunocompetent patients, primary infection is usually asymptomatic or presents as a mononucleosis syndrome that may be associated with hepatic cytolysis. Diagnosis is based on serology. Reactivation of the latent virus is possible and usually goes unnoticed. In immunocompromised patients, primary infection and reactivation may be responsible for serious local or widespread infections (pneumopathy, hepatitis, colitis, encephalitis, retinitis). Diagnosis of CMV infection and disease is based on detection of the viral genome in blood and/or in a sample from the affected organ (BAL, CSF, biopsy, aqueous humour). CMV is the main cause of congenital infection, leading to foetal death in utero, malformations and intrauterine growth retardation. The risk of congenital anomaly is increased when the primary infection occurs before 20 weeks of amenorrhoea. Diagnosis of maternal infection is based on serology. However, the virus is not systematically transmitted to the foetus. If a recent maternal CMV infection is diagnosed and/or there are ultrasound findings suggestive of congenital CMV infection, an amniocentesis may be performed to test the amniotic fluid for the presence of the viral genome. Prenatal diagnosis must always be confirmed (or invalidated) during the first three weeks of life by testing for the virus in urine or saliva. At birth, 90% of infected newborns are asymptomatic. They may, however, develop auditory and cognitive consequences later in life, requiring long-term monitoring.

Preanalytics

•  :
• Whole blood or plasma (EDTA), serum, urine only in infants < 3 weeks: Quantitative result Bronchial aspirations, BAL, aqueous humor, biopsies (including digestive, pulmonary, cerebral), puncture fluid (pleural or pericardial), bone marrow (EDTA), CSF, saliva only in infants < 3 weeks: Qualitative result
•   Reftrigerated

• A tube specifically for this analysis : No

Further information

.
[To be translated]
The use of the S14UK transport bag is Mandatory.
Swab samples require the use of a transport medium (viral) supplied in kit K1.
Samples (other than swabs) must be sent as they are, in sterile bottles, without transport medium.

Specific equipment available

• S14: Special mycobacteria transport bags are to be used

Documents to download

• information note (N23-INTGB)

Methodology

Real-time PCR

Turnaround time

3 days