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Whooping cough - Bordetella pertussis and parapertussis - direct diagnosis - PCR

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Eurofins Biomnis code

COQBM

Synonyms

  • Bordetella pertussis
  • Bordet-Gengou Bacillus

Clinic significance

This is a respiratory infection caused by Bordetella pertussis, characterised by episodes of coughing fits, which can be serious in infants. Vaccination has brought the disease under relative control, but protection is not absolute. Older children, adolescents and adults may present with pertussis, which is often atypical and contributes to the spread of the germ. In practice, bacteriological diagnosis is difficult because of the fragility of the bacteria. Detection by gene amplification (PCR) is preferable. PCR should be carried out no later than 3 weeks after the onset of clinical signs.

Prenalytics

  • Endonasal or nasopharyngeal swab, nasopharyngeal aspiration, bronchial aspiration
  • Refrigerated

Further information

  • The use of the S14UK transport bag is Mandatory.
  • The test is not relevant if cough is persisting for more than 3 weeks
  • .
  • Samples (other than swabs) must be sent as they are, in sterile bottles, without transport medium.
  • Swab samples require the use of a transport medium (viral) supplied in kit K1.

Specific equipment available

K1: Virus screening kit by culture or PCR

Documents to download


Methodology

Real-time PCR

Turnaround time

2 days


Pathologist(s) in charge
Biomnis Lyon
Specialty
Infectious
Contact(s)
Dr Anaïs SOARES
Dr Marie HERVO
Dr Secrétariat médical spécialisé Eurofins Biomnis
Dr Thomas BLANCHOT
Phone(s)
+334 72 80 73 99
+334 72 80 73 99
+334 72 80 73 99
+334 72 80 73 99