Whooping cough - Bordetella pertussis and parapertussis - direct diagnosis - PCR
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COQBM
Synonyms
- Bordetella pertussis
- Bordet-Gengou Bacillus
Clinic significance
This is a respiratory infection caused by Bordetella pertussis, characterised by episodes of coughing fits, which can be serious in infants. Vaccination has brought the disease under relative control, but protection is not absolute. Older children, adolescents and adults may present with pertussis, which is often atypical and contributes to the spread of the germ. In practice, bacteriological diagnosis is difficult because of the fragility of the bacteria. Detection by gene amplification (PCR) is preferable. PCR should be carried out no later than 3 weeks after the onset of clinical signs.
Prenalytics
- Endonasal or nasopharyngeal swab, nasopharyngeal aspiration, bronchial aspiration
- Refrigerated
Further information
- The use of the S14UK transport bag is Mandatory.
- The test is not relevant if cough is persisting for more than 3 weeks
- .
- Samples (other than swabs) must be sent as they are, in sterile bottles, without transport medium.
- Swab samples require the use of a transport medium (viral) supplied in kit K1.
Specific equipment available
K1: Virus screening kit by culture or PCR
Documents to download
Methodology
Real-time PCR
Turnaround time
2 days
Pathologist(s) in charge
Biomnis Lyon
Specialty
Infectious
Contact(s)
Dr Anaïs SOARES
Dr Marie HERVO
Dr Secrétariat médical spécialisé Eurofins Biomnis
Dr Thomas BLANCHOT
Dr Marie HERVO
Dr Secrétariat médical spécialisé Eurofins Biomnis
Dr Thomas BLANCHOT
E-mail(s)
Phone(s)
+334 72 80 73 99
+334 72 80 73 99
+334 72 80 73 99
+334 72 80 73 99
+334 72 80 73 99
+334 72 80 73 99
+334 72 80 73 99