Helicobacter pylori and clarithromycin resistance gene - direct diagnosis - PCR
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HPGEN
Synonyms
- Clarithromycin
- gène de résistance à la clarithromycine-PCR
- H.pylori
- Macrolides
- 23S
Specialty
Infectious
Clinical significance
Helicobacter pylori is a bacterium (a Gram-negative, helical-shaped, microaerophilic bacillus) that can cause gastritis, peptic ulcer disease and, more rarely, gastric adenocarcinoma or MALT lymphoma. In many cases, the disease is asymptomatic. Transmission is human-to-human, oral-to-oral, often intra-familial, in early childhood. H. pylori is a fragile bacterium, and culture is sometimes unsuccessful for a variety of reasons (pre-analytical conditions, previous antibiotic treatment, etc.). PCR detection of H. pylori from gastric biopsies is much faster and more sensitive than culture. PCR is now the 1st-line technique for diagnosing H. pylori infection, in the absence of eradication failure. PCR can also be used to identify the main mutations responsible for resistance to clarithromycin by detecting mutations in the 23S gene. If a clarithromycin resistance mutation is detected, a culture will be carried out from the gastric biopsy and antibiotics' MICs may be determined as well.
Further information
The use of the S14UK transport bag is Mandatory.
Biopsies should be sent in a transport medium for Helicobacter pylori or as they are in a sterile vial.
Refrigerated if <= 72 h / frozen if > 72 h
En réfrigéré si délai <= 72 h / congelé si délai > 72h
Specific equipment available
- T8 : Helicobacter pylory (culture & transport media)
Documents to download
Methodology
Real-time PCR
Turnaround time
7 days
