Eurofins Biomnis code

IPAL

Synonyms

• ALP - isoenzymes
• electrophoresis of alkaline phosphatases
• Isoenzymes - alkaline phosphatase
• [To be translated]

Specialty

Biochemistry

Clinical significance

Alkaline phosphatase (ALP) is a metalloglycoprotein which occurs in many different tissues, including liver, bone, the kidneys, the intestine and the placenta. There are three different ALP genes: the non-tissue-specific gene, the intestinal gene and the placental gene. It is the product of the non-tissue-specific gene which is modified to create the enzymes typical of each tissue.In the normal situation, three fractions are present, hepatic, bone and intestinal fractions. In pre-adolescent children, it is the bone enzyme which predominates (up to 90%). The placental isoenzyme appears in the mother's serum in the fourth month of pregnancy and persists until delivery.Insofar as pathology is concerned, a major increase in bone enzyme activity has been demonstrated in Paget's disease and vitamin D-dependent rickets. An extra hepatobiliary H2 fraction appears in intra- or extra-hepatic cholestasis and in hepatic metastasis. Abnormal production of the placental isoenzyme (the Regan isoenzyme) is associated with certain lung and genitourinary malignancies, including ovarian and cervical cancer. Appearance of the placental-like isoenzyme is associated with testicular cancer and levels of the fetal intestinal isoenzyme rise in primary liver cancer.

Preanalytics

• 2 mL :
• serum (do not use 10 mL tube)
•   Reftrigerated

• A tube specifically for this analysis : No

Further information

Not to to be performed on haemolysed sample

Methodology

Electrophoresis

Turnaround time

2 days