Kingella kingae - direct diagnosis - PCR

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Eurofins Biomnis code

KINGK

Specialty

Infectious


Clinical significance

Kingella kingae is a commensal bacterial species of the oropharynx. It is a Gram-negative cocco-bacillus. K. kingae is the bacterium most frequently responsible for osteoarticular infections in children under the age of 4: septic arthritis (mainly of the lower limbs) and, more rarely, osteomyelitis. In a child aged between 6 and 48 months, osteoarticular pain, functional impotence or localised oedema, in the absence of trauma, should raise the suspicion of a K. kingae osteoarticular infection. More rarely, K. kingae may be responsible for isolated bacteremia, endocarditis (HACEK group endocarditis [acronyms for subacute endocarditis caused by fastidious, slow-growing microorganisms]) or spondylodiscitis. Kingella kingae can be detected by culture (from a sample of joint fluid, sometimes inoculated into blood culture bottles). Specific PCR testing can be used to confirm the diagnosis, particularly if the culture is negative or if antibiotic therapy was started before the joint fluid was punctured.

Preanalytics
  • A tube specifically for this analysis : No
Further information

[To be translated]
The use of the S14UK transport bag is Mandatory.
Swab samples require the use of a transport medium (viral) supplied in kit K1.
Samples (other than swabs) must be sent as they are, in sterile bottles, without transport medium.

Specific equipment available
  • S14: Special mycobacteria transport bags are to be used
Documents to download

Methodology

Real-time PCR

Turnaround time

6 days


Testing Laboratory

Biomnis Lyon