Eurofins Biomnis code

LEPBM

Specialty

Infectious

Clinical significance

Leptospirosis is a ubiquitous zoonosis that predominates in warm and humid regions. It is caused by a bacterium of the genus Leptospira, which includes 10 species pathogenic to humans and animals (rodents and domestic pets). Transmission occurs through contact with soil and water contaminated by the urine of infected animals, or through direct contact. Clinical expression varies, from a mild flu-like form to a severe form with multi-visceral damage, sometimes associated with haemorrhagic manifestations. Biological diagnosis is based on PCR and serology. Detection of the Leptospira genome is possible in blood, CSF or urine, depending on the stage of the disease. During the first week of the disease, leptospires spread throughout the body via the bloodstream. During the second week, the germs disappear from the blood and are present in the CSF and urine. Antibodies appear at the end of the first week of the disease. Leptospirosis is a reportable disease.

Preanalytics

•  :
• Whole blood or plasma (EDTA), serum, urine, CSF
•   Reftrigerated

• A tube specifically for this analysis : No

Further information

.
PCR on blood samples should only be carried out during the viremic phase (in the first ten days after the onset of the disease).
The use of the S14UK transport bag is Mandatory.

Documents to download

• information note (N23-INTGB)

Methodology

Real-time PCR

Turnaround time

3 days