Eurofins Biomnis code

MOHC2

Synonyms

• acquired molecular cytogenetics
• Acquired FISH

Clinic significance

Probes Blood :
- 1p19q
- 1p1q
- 20q
- BCL6
- BCR/ABL
- CBFB
- CEP7/7q
- CEPX/Y
- cMYC
- EGR1
- ETV6/RUNX1 (TEL/AML1)
- EVI1 (MECOM)
- FGFR1/CEP8
- FIP1L1/PDGFRA
- IGH DC
- IGH/BCL2
- IGH/CCND1
- IGH/FGFR3
- IGH/MAF
- Kit LLC : TP53, ATM, CEP12, 13q
- MALT1
- MLL(KMT2A)
- nMYC
- NUP98
- P16/CEP9
- PDGFRB
- PML/RARA
- RARA DC
- RUNX1/RUNX1T1 (ETO/AML1)
- TCF3/PBX1
- TCRA/D
- TP53/CEP17

Prenalytics

• 5 mL Heparinised whole blood, 2 mL Bone marrow, lymph nodes
• Ambient temperature

Further information

• Bone marrow: 1 heparinised syringe or medullary puncture transferred into a lithium heparinate tube ,Lymph node: sterile bottle
• ALWAYS attach:
  -The clinical and therapeutic details, full blood count results, platelets levels
  The bone marrow report
  Immunophenotyping report
  The result of the Karyotype performed at the diagnostic step if not performed in Biomnis
• Cytogenetic study in the context of Myeloma: must be performed on bone marrow (not on peripheral blood). Exception: Plasma cell leukaemia: peripheral blood or bone marrow.

Specific equipment available

S9L: Envelopes yellow for karyotypes LYON

Documents to download

• Specific request form B8-INTGB
• DS2-INTFR - Pathological diagnosis and follow up of patients with Chronic Myeloid Neoplasms
• DS3 -INTGB - Acute leukemia
• DS62- Mature lymphoid noeplasm

Methodology

Molecular cytogenetics or FISH

Turnaround time

2 weeks if isolated FISH request, otherwise according to indication (LA: 1 week, SMP and MDS: 2 weeks, SLP and myeloma: 3 weeks)