Eurofins Biomnis code

PARBM

Synonyms

• 5ème maladie éruptive de l'enfant

Specialty

Infectious

Clinical significance

Parvovirus B19 is a DNA virus. In children, it causes an eruptive disease known as erythema infectiosum or megalerythema epidemic, the 5th most common childhood eruptive disease. In adults, late-onset primary infection may be accompanied by joint symptoms (polyarthralgia). The virus has a tropism for precursors of the erythrocyte lineage. In predisposed individuals with a constitutional haemolytic anaemia, Parvovirus B19 infection causes an acute erythroblastopenia crisis.Virological diagnosis of B19 virus infection is used primarily in severe or complicated forms. It is not generally recommended for the diagnosis of epidemic megalaemia. Diagnosis of primary infection with Parvovirus B19 is most often based on serology (in immunocompetent patients). In case of an acute erythroblastopenia crisis or chronic anaemia in immunocompromised patients, detection of the virus DNA in the blood, and possibly in the bone marrow, is the technique of choice. Measurement of the viral load in peripheral blood can be useful in differentiating between acute and frequent persistent infections, and can also be useful in the early phase of acute PRCA crisis.After maternal-foetal transmission, parvovirus B19 can induce profound anaemia associated with viral myocarditis, which can be complicated by foeto-placental hydrops. Proof of transplacental passage is obtained by detecting viral DNA in the amniotic fluid taken by amniocentesis.

Preanalytics

• 1 mL :
• bone marrow (EDTA tube)
•   Reftrigerated

• A tube specifically for this analysis : No

Further information

Biopsies should be placed as they are, in a dry tube .
The use of the S14UK transport bag is Mandatory.
The following samples are not accepted: placenta, umbilical cord or any other sample of foetal origin (except blood).

Methodology

Real-time PCR

Turnaround time

6 days