Eurofins Biomnis code

PCRUN

Synonyms

• ARN ribosomal 16S
• DNA bacterien
• Next Generation Sequencing
• NGS
• panbacterien
• universal PCR
• 16S rRNA

Specialty

Infectious

Clinical significance

16S PCR is a molecular biology technique used to detect and identify one or more bacterial strains from a biological sample. 16S PCR is ¿universal' because it targets a genomic sequence common to all bacteria: a gene coding for 16S ribosomal DNA. The technique uses 16S detection PCR to amplify the gene and sequence the PCR product using Next Generation Sequencing (NGS) technology. NGS technology makes it possible to simultaneously identify several bacteria present in a biological sample. 16S PCR can be performed directly on clinical samples from sites that are normally sterile (puncture fluids, heart valves, organ biopsies, etc.), as well as on isolated bacterial strains. This technique is particularly useful for identifying slow-growing bacteria that are difficult or impossible to culture. It is also indicated in situations where patients have been treated with antibiotics prior to sampling. Because of the sensitivity of universal PCR techniques, a negative 16S PCR result does not definitively rule out infection. However, the sensitivity of a specific PCR is greater than that of a universal PCR. In cases where there is a strong clinical presumption of infection by a specific bacterial species or genus, targeted research using a specific PCR should therefore be the first choice. The prescription for this test must be accompanied by the patient's clinical and therapeutic context (request form B18).

Preanalytics

• 0.5 mL (pour les prélèvements liquides, flacon stérile) :
• Puncture fluids (joint fluid, ascites fluid, pericardial fluid, pleural fluid), osteoarticular samples, heart valve, deep eye samples (aqueous humor, vitreous humor), biopsy, CSF, bacterial strain
•   Refrigerated

• A tube specifically for this analysis : Yes

Further information

Delivery time :
4h at room temperature (+15°C to +25°C) before dispatch
5 days refrigerated from date of sampling to arrival at our laboratory
1 month frozen at -20°C between sampling date and arrival at our laboratory
The use of the S14UK transport bag is Mandatory.
For biological fluids such as puncture fluids and osteoarticular samples: use an airtight, sterile container adapted to the volume. Grindings are acceptable.
For biopsies, do not add liquid to the sterile bottle.
Transport media and swabs are not accepted.

Specific equipment available

• S14: Special mycobacteria transport bags are to be used

Documents to download

• Specific request form (B18-INTGB)

Methodology

NGS sequencing

Turnaround time

9 days