Eurofins Biomnis code

RAI

Synonyms

• Anti-erythrocyte antibodies - screening/identification
• IAT - screening/identification
• Indirect Coombs

Specialty

Haematology

Clinical significance

This test is performed in the context of predicting and preventing post-transfusion reactions and problems which could arise as a result of fetomaternal alloimmunization. The presence in a blood transfusion of antibodies which give a positive result in an indirect antiglobulin test (37 °C) could have grave consequences. If alloantibodies are detected, the erythrocytes should be phenotyped to confirm that the relevant antigen is not expressed on the subject's cells.

Preanalytics

• 2 x 5 mL :
• EDTA whole blood (do not use tubes with separator gel)
•   Refrigerated

• A tube specifically for this analysis : No

Further information

Enclose the specific clinical information form (R3-INTGB : Immunology-haematology (IA))
[To be translated]
Please specify the patients full civil status on the request form and primary tube: maiden name, married name or surname used, full first name, gender and date of birth. Please send us the primary tube.
1 individual aliquot for this analysis.
[To be translated]
SUPPLEMENTARY ANALYSES IN THE EVENT OF BIOLOGICAL ANOMALY DETECTION: when performing specific analyses, the biologists complete the evaluation in order to characterise or have a better typing of a biological anomaly, we perform:
- identification which is compulsory if the screening test is positive,
- the RH-KEL1 phenotypes and/or a phenotype extended to cw, Kidd, Duffy antigens, etc. which are systematic, failing a valid result, to validate the anti-erythrocyte antibody identification,
- an anti-erythrocyte antibody titration other than anti-A, anti-B with an assay by weight for anti-RH, which are compulsory for all pregnant women in whom we have identified an immune antibody,
- a direct Coombs test to screen for antibodies fixed on red blood cells,
- an adsorption test in the event of antibody mixing or autoimmunisation

Contact the International Division for further information

Methodology

Indirect antiglobulin test on gel-filtration medium and/or microplate

Turnaround time

1 days (2 days in case of anti D titration/microtitration and 2 weeks in case sample is referred to the National Reference centre for results confirmation)