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Indirect antiglobulin test - IAT - screening/identification

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Eurofins Biomnis code

RAI

Synonyms

  • Anti-erythrocyte antibodies - screening/identification
  • IAT - screening/identification
  • Indirect Coombs
  • Anti-erythrocyte antibodies - screening/identification

Clinic significance

This test is performed in the context of predicting and preventing post-transfusion reactions and problems which could arise as a result of fetomaternal alloimmunization. The presence in a blood transfusion of antibodies which give a positive result in an indirect antiglobulin test (37 °C) could have grave consequences. If alloantibodies are detected, the erythrocytes should be phenotyped to confirm that the relevant antigen is not expressed on the subject's cells.

Prenalytics

  • 2 x 5 mL
  • EDTA whole blood (do not use tubes with separator gel)
  • Refrigerated

Further information

  • 1 individual aliquot for this analysis.
  • SUPPLEMENTARY ANALYSES IN THE EVENT OF BIOLOGICAL ANOMALY DETECTION: when performing specific analyses, the biologists complete the evaluation in order to characterise or have a better typing of a biological anomaly, we perform:
    - identification which is compulsory if the screening test is positive,
    - the RH-KEL1 phenotypes and/or a phenotype extended to cw, Kidd, Duffy antigens, etc. which are systematic, failing a valid result, to validate the anti-erythrocyte antibody identification,
    - an anti-erythrocyte antibody titration other than anti-A, anti-B with an assay by weight for anti-RH, which are compulsory for all pregnant women in whom we have identified an immune antibody,
    - a direct Coombs test to screen for antibodies fixed on red blood cells,
    - an adsorption test in the event of antibody mixing or autoimmunisation

    Contact the International Division for further information
  • Please specify the patients full civil status on the request form and primary tube: maiden name, married name or surname used, full first name, gender and date of birth. Please send us the primary tube.
  • Enclose the specific clinical information form (R3-INTGB : Immunology-haematology (IA))
  • [To be translated]
  • [To be translated]

Methodology

Indirect antiglobulin test on gel-filtration medium and/or microplate

Turnaround time

1 days (2 days in case of anti D titration/microtitration and 2 weeks in case sample is referred to the National Reference centre for results confirmation)


Pathologist(s) in charge
Biomnis Paris
Specialty
Haematology
Contact(s)
Dr Léna LE FLEM
Dr Bahya AMEYOUD
Dr Nihad MEKNACHE
Phone(s)
+331 49 59 17 44
+331 49 59 17 08
+331 49 59 63 40