Eurofins Biomnis code

RMGEN

Synonyms

• Mycoplasma genitalium
• Resistance to macrolides

Specialty

Molecular biology

Clinical significance

Mycoplasma genitalium is a globally distributed, sexually transmitted intracellular bacterium responsible for 10 to 35% of acute or chronic nongonococcal urethritis (NGU). In women, M. genitalium is the only mycoplasma responsible for cervicitis. It would also be responsible for endometritis, salpingitis, spontaneous abortions, premature births and could be associated with female infertility. M. genitalium infections can be asymptomatic. Detection of M. genitalium should only be done by nucleic acid amplification techniques (NAAT) and for symptomatic patients. In recent years, acquired resistance to macrolides, first-line antibiotics, has increased sharply worldwide. This resistance reached nearly 35% in France in 2019. It is therefore recommended to test for macrolide resistance on each positive sample for M. genitalium. We will propose the Search for mutations associated with macrolide resistance, with the ResistancePlus® MG kit (SpeeDx), allowing the detection of 4 mutations at positions 2058 and 2059 in the 23S rRNA gene. This analysis can be done on the media used to detect M. genitalium

Preanalytics

• 1 mL :
• Urine, Vaginal sampling, Vaginal self-sampling
•   Ambient temperature

• A tube specifically for this analysis : No

Further information

K12: Aptima Urine Collection Kit for urine samples
If APTIMA K12 medium is not available: Send a sterile urine jar without additives.
PROHIBIT jars with integrated transfer needle or cannula.
K14: Aptima Multi-Test Swab Collection Kit for vaginal and self-collection of vaginal, anal and pharyngeal samples.
Avoid pots with integrated needles or transfer cannulas
Banning NEST environments
The sample is stable for 15 days at room temperature or refrigerated.

Methodology

PCR

Turnaround time

3 days