Eurofins Biomnis code

ROUBM

Synonyms

• Morbillivirus
• Rougeole

Specialty

Infectious

Clinical significance

Measles is a highly contagious, reportable eruptive disease caused by a virus of the genus Morbillivirus. It presents as a maculopapular rash associated with fever, asthenia and oculo-respiratory signs (cough, conjunctivitis, rhinitis). Since vaccination was introduced, the number of cases has fallen considerably. Most vaccinated patients are asymptomatic, but cases have been described in previously immunised patients. Diagnosis is based on the search for the viral genome in an oropharyngeal sample and on serology, but the latter is difficult to interpret if the patient has previously been vaccinated. The optimum period for detecting the virus in the oropharynx is between D0 and D5 after the onset of the rash. In active epidemic outbreaks, biological confirmation of the diagnosis is not mandatory. The measles virus causes serious neurological and pulmonary complications. Complicated forms are more common in people under 1 year old (pneumopathy), over 20 (neuropathy), immunocompromised people and pregnant women. If pneumonia is suspected, it is possible to test for the virus in bronchial secretions or BAL, although few studies have been published to date on the presence of the virus in this type of sample. A search for the viral genome may be carried out in CSF or urine, together with a search for intra-thecal synthesis of anti-measles IgG in cases of suspected acute post-infectious encephalitis or SSPE (subacute sclerosing panencephalitis). The diagnosis of inclusion encephalitis is based on a search for the viral genome on a brain biopsy of the injured area using imaging (the virus cannot be found elsewhere).

Preanalytics

• Saliva, Oropharynx, Nasopharynx, bronchial aspiration, BAL, CSF, urine
•   Refrigerated

• A tube specifically for this analysis : No

Further information

[To be translated]
The use of the S14UK transport bag is Mandatory.
.
Swab samples require the use of a transport medium (viral) supplied in kit K1.
Samples (other than swabs) must be sent as they are, in sterile bottles, without transport medium.

Specific equipment available

• S14: Special mycobacteria transport bags are to be used

Documents to download

• information note (N23-INTGB)

Methodology

Real-time PCR

Turnaround time

1 day