Eurofins Biomnis code

RPC

Synonyms

• Activated protein C resistance test
• Dahlback test
• RPCa
• Dahlback factor

Clinic significance

Activated Protein C Resistance (APCR, also referred to as Factor V Leiden) is an important risk factor for familial thrombophilia. This phenomenon is found in 15 to 25% of subjects with a history of venous thromboembolism and in 2 to 7% of the population as a whole. In 90 to 95% of cases, this abnormality is associated with a specific mutation in the gene encoding Factor V (a Gln to Arg transition at position 1691 - Arg506Gln); the mutant Factor V (also referred to as Factor V Leiden) is resistant to inactivation by Protein C. There are currently two different modalities for diagnosing this condition. The first is a functional test based on detecting a prolonged Activated Partial Thromboplastin Time after addition of activated Protein C with Factor V-deficient plasma and a heparin inhibitor. The second is based on molecular genetic methods to detect the actual mutation in the subject's genome.

Prenalytics

• 2 mL
• Citrated Plasma
• FROZEN

Further information

• It is advisable to send us a frozen and centrifuged citrate sample in compliance with the GFHT pre-analytical recommendations.
• Please specify whether factor V Leiden should be performed if the activated protein C resistance test is positive (please ensure to enclose a consent form from the patient in accordance with the regulatory requirements, as well as 5 mL EDTA whole blood)
• [To be translated]

Methodology

Chronometric method

Turnaround time

2 days