Eurofins Biomnis code

COLO1

Synonyms

• companion test
• NGS
• oncologt
• solid tumor
• targeted therapy

Specialty

Genetics

Clinical significance

The NGS "COLO1" panel fulfils the routine needs of clinicians for prescribing targeted therapies for metastatic colorectal cancer. The KRAS, NRAS and now also BRAF statuses can be used to guide the selection of a targeted therapy (monoclonal anti-EGFR Ab and anti-BRAF Ab). This panel is complemented by the MSI test, screening for MLH1 methylation and COLO-RECTAL FISH unit tests.

Preanalytics

• Tumoral block included in paraffin or slides (6 slides 5µm) or DNA extract
•   Ambient temperature

• A tube specifically for this analysis : No

Further information

Use the specific request form B9-INTGB: Oncology-Solid tumors
The use of the S25UK transport bag is Mandatory.
Attach the anatomopathological report

Specific equipment available

• S25 : Envelope Genetics and Molecular Oncology

Documents to download

• Specific form (B9-INTGB)
• Management of solid tumours in Biopathology (DS5-INTGB)
• Digestive system Panel form (DS84-INTGB)

Methodology

Targeted sequencing of cancer genes (NGS) : -Library : AmpliSeq Library PLUS for Illumina Custom Panel V1 - Amplicon-based targeted library preparation method -NGS Platform : MiSeq Illumina - Flowcell : microV2 (2x150pb) - Paired-end reads -Analysis software : Miseq control Software version 2.6.2.1 /SeqOne Platform v1.2 - CE Pipeline version : SomaVar v1.4 CE -Limit of detection : 5% -Minimum depth accepted : 500x -Human genome version : Hg19

Turnaround time

10 business days