NGS Panel - Solid Tumor - GIST: BRAF,KIT,PDGFRA

Back to the list
Eurofins Biomnis code

GIST

Synonyms
  • companion test
  • NGS
  • oncology
  • solid tumor
  • targeted therapy
Specialty

Genetics

Clinical significance

Testing for KIT, PDGFRA and BRAF mutations thus has diagnostic and/or prognostic and/or theranostic value.

Preanalytics
  • Tumoral block included in paraffin or slides (6 slides 5µm) or DNA extract or Shavings (6 shavings of 5 µm)
  •   Ambient temperature
  • A tube specifically for this analysis : No
Further information

Use the specific request form B9-INTGB: Oncology-Solid tumors
The use of the S25UK transport bag is Mandatory.
Attach the anatomopathological report

Specific equipment available
  • S25 : Envelope Genetics and Molecular Oncology
Documents to download
Methodology

Search for variants on a targeted gene panel using high-throughput sequencing. Library preparation: Library Preparation Enzymatic Fragmentation Kit 2.0 Twist with UMI Capture-based enrichment (custom panel) Sequencing: NovaSeq Illumina (2x150bp) Bioinformatic analysis: Demultiplexing with BCLconvert / SeqOne SomaVar v2.7 RUO Theoretical detection threshold: 5% Minimum depth: 99% coverage at 200X (after UMI analysis) for Level I and II genes relevant to the indication, according to the 2024 ESMO recommendations (PMID: 38834388). >95% coverage at 200X for all other genes. Genome version: GRCh38

Turnaround time

15 days

Testing Laboratory

Biomnis Lyon

Contact(s)
Customer Care