Eurofins Biomnis code

MELA

Synonyms

• companion test
• NGS
• oncology
• solid tumor
• targeted therapy

Specialty

Genetics

Preanalytics

• Tumoral block included in paraffin or slides (6 slides 5µm) or DNA extract or Shavings (6 shavings of 5 µm)
•   Ambient temperature

• A tube specifically for this analysis : No

Further information

Use the specific request form B9-INTGB: Oncology-Solid tumors
The use of the S25UK transport bag is Mandatory.
Attach the anatomopathological report

Specific equipment available

• S25 : Envelope Genetics and Molecular Oncology

Documents to download

• Specific form (B9-INTGB)
• Management of solid tumours in Biopathology (DS5-INTGB)
• Other solid tumours (DS86-INTGB)

Methodology

Search for variants on a targeted gene panel using high-throughput sequencing. Library preparation: Library Preparation Enzymatic Fragmentation Kit 2.0 Twist with UMI Capture-based enrichment (custom panel) Sequencing: NovaSeq Illumina (2x150bp) Bioinformatic analysis: Demultiplexing with BCLconvert / SeqOne SomaVar v2.7 RUO Theoretical detection threshold: 5% Minimum depth: 99% coverage at 200X (after UMI analysis) for Level I and II genes relevant to the indication, according to the 2024 ESMO recommendations (PMID: 38834388). >95% coverage at 200X for all other genes. Genome version: GRCh38

Turnaround time

15 days