NGS Panel - Solid Tumor - PAN: AKT1 - ALK - AR - ATM - BARD1 - BRAF - BRCA1 - BRCA2 - BRIP1 - CDK12 - CDK4 - CDK6 - CDKN2A - CHEK1 - CHEK2 - CTNNB1 - DDR2 - EGFR - ERBB2 - ESR1 - EZH2 - FANCA - FANCL - FBXW7 - FGFR1 - FGFR2 - FGFR3 - FOXL2 - GNA11 - GNAQ - GNAS - HRAS - IDH1 - IDH2 - JAK2 - KEAP1 - KIT - KRAS - MAP2K1 - MET - MLH1 - MRE11 - NBN - NRAS - NTRK1 - PALB2 - PDGFRA - PIK3CA - POLE - PPP2R2A - PTEN - RAD51B - RAD51C - RAD51D - RAD54L - RAF1 - RB1 - RET - ROS1 - SF3B1 - SMAD4 - STK11 - TERT - TP53

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Eurofins Biomnis code

PAN

Synonyms
  • companion
  • NGS
  • oncology
  • solid tumor
  • targeted therapy
Specialty

Genetics

Preanalytics
  • Tumoral block included in paraffin or slides (6 slides 5µm) or DNA extract or Shavings (6 shavings of 5 µm)
  •   Ambient temperature
  • A tube specifically for this analysis : No
Further information

Use the specific request form B9-INTGB: Oncology-Solid tumors
The use of the S25UK transport bag is Mandatory.
Attach the anatomopathological report

Specific equipment available
  • S25 : Envelope Genetics and Molecular Oncology
Documents to download
Methodology

Search for variants on a targeted gene panel using high-throughput sequencing. Library preparation: Library Preparation Enzymatic Fragmentation Kit 2.0 Twist with UMI Capture-based enrichment (custom panel) Sequencing: NovaSeq Illumina (2x150bp) Bioinformatic analysis: Demultiplexing with BCLconvert / SeqOne SomaVar v2.7 RUO Theoretical detection threshold: 5% Minimum depth: 99% coverage at 200X (after UMI analysis) for Level I and II genes relevant to the indication, according to the 2024 ESMO recommendations (PMID: 38834388). >95% coverage at 200X for all other genes. Genome version: GRCh38

Turnaround time

15 days

Testing Laboratory

Biomnis Lyon

Contact(s)
Customer Care