Eurofins Biomnis code

TRABM

Synonyms

• [To be translated]

Specialty

Infectious

Clinical significance

Bacterium which only infects the eyes and the genitalia. It usually causes trachoma, inclusion conjunctivitis or sexually transmitted disease. Lymphogranuloma venereum remains an exotic infection. "Non-specific" urethritis and cervicitis can be relatively asymptomatic and lead to spread of the infection and ultimately tubular damage and infertility in women or epididymitis and prostatitis in men. A role for C. trachomatis has been suggested in some forms of perihepatitis and in Reiter's syndrome. Infants born to infected mothers may present with post-natal conjunctivitis (which is resistant to silver nitrate treatment) and severe pneumonia. The bacterium can often be directly detected in conjunctival or genital smears. Nucleic acid-based techniques (e.g. PCR) are sensitive, specific and do not necessitate invasive procedures (a urine specimen or a vulvar smear is suitable). Culture takes much longer and requires a urethral, cervical or conjunctival smear and perfect transport conditions. Seroconversion and the presence of IgM are demonstrated in cases of recent primary infection and in infected newborns. Apart from these situations, the presence of IgG indicates contact with the bacterium, which cannot be dated, in that these antibodies persist for a long time and are only influenced by an effective treatment at a late stage. The presence of specific IgA may be of interest in cases of active infection. Note the existence of serological cross-reactions between the different Chamydia species.

Preanalytics

• Sample on Aptima collection kit (Hologic): Kit K11 : cervical, urethral, vaginal, conjonctival swab, biopsy, IUD. For liquid samples (200 µL minimum): sperm, Douglas pouch fluid, Bartollin fluid, peritoneal fluid Kit K14: self-sampling (vaginal), samples and self-sampling (anal,pharyngeal) - Kit K12: urine from the 1st jet taken at least 1 hour after the last micturition.
•   Ambient temperature

• A tube specifically for this analysis : No

Further information

K11: Aptima Unisex Swab Collection Kit for endocervical and urethral swabs
K12: Aptima Urine Collection Kit for urine samples or sterile urine jar without additives.
PROHIBIT jars with integrated transfer needle or cannula.
K14: Aptima Multi-Test Swab Collection Kit for vaginal and self-collection of vaginal samples.

Documents to download

• Sampling protocol (K11P-INTGB)
• Sampling protocol (K12P-INTGB)
• Sampling protocol (K14P-INTGB)

Methodology

Molecular biology. Technique: Eurobio Scientific (real-time PCR)

Turnaround time

2 days